Matthew R Sarkisian

Matthew R Sarkisian,

Associate Professor, Program Director MS In Neuroscience Graduate Program

Department: Department of Neuroscience
Business Phone: (352) 273-5534
Business Email: msarkisian@ufl.edu

Teaching Profile

Courses Taught
2011-2024
GMS6021 Organization and Development of the Nervous System
2017-2021
GMS6705 Functional Human Neuroanatomy
2019,2021-2023,2023-2024
GMS6013 Developmental Genetics
2018
GMS7980 Research for Doctoral Dissertation
2015
GMS7794 Neuroscience Seminar
2011,2021-2024
GMS6073 Disorders of the Developing Nervous System
2010,2023
GMS7795 Special Topics in Neuroscience
2022-2024
GMS6007 Fundamentals of Neuroscience
2021-2024
GMS6029 Brain Journal Club
2022-2023
GMS6905 Independent Studies in Medical Sciences
2022-2023
GMS6701 Functional and Comparative Neuroanatomy for Professionals
2023
GMS6971 Research for Master’s Thesis
2023
BMS6020 Clinical Neuroscience

Research Profile

The goal of my research program here at the University of Florida Dept of Neuroscience is to understand the cellular and molecular mechanisms that contribute to both the normal and abnormal growth of the cerebral cortex. When this process goes awry, it is believed to underlie a host of neurodevelopmental disorders (e.g. mental retardation, epilepsy, autism, intellectual disability) and potentially diseases that manifest with age (e.g. neurodegenerative, brain cancer). My laboratory is particularly intrigued by primary cilia, hair like organelles with highly sophisticated sensory and signaling capacities found on virtually all neural cell types, and how they contribute to cerebral cortical growth and function. One line of research is examining how cilia influence the development and function of cortical neurons. We use in utero and neonatal gene transfer to manipulate and track the fate, morphology and function of neurons with normal and abnormal cilia. We also use biochemical, molecular, cell culture, electron microscopic and various histological methods to assist in these analyses. A second line of research in the lab is investigating how primary cilia influence the pathogenesis of human glioblastoma, one of the most common and feared brain tumors in adult. In collaboration with other MBI members and the Florida Center for Brain Tumor Research, we use primary tumor cell lines, xenograft models and patient biopsies to examine how cilia impact tumor growth and resistance to current treatments.

Open Researcher and Contributor ID (ORCID)

0000-0002-0566-6480

Publications

2024
Aurora kinase A inhibition plus Tumor Treating Fields suppress glioma cell proliferation in a cilium-independent manner
Translational Oncology. 45 [DOI] 10.1016/j.tranon.2024.101956. [PMID] 38640786.
2024
KR158 spheres harboring slow-cycling cells recapitulate GBM features in an immunocompetent system.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2024.01.26.577279. [PMID] 38501121.
2023
Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia.
Cells. 12(19) [DOI] 10.3390/cells12192354. [PMID] 37830570.
2023
Superenhancer activation of KLHDC8A drives glioma ciliation and hedgehog signaling.
The Journal of clinical investigation. 133(2) [DOI] 10.1172/JCI163592. [PMID] 36394953.
2022
Heterogeneity of glioblastoma stem cells in the context of the immune microenvironment and geospatial organization.
Frontiers in oncology. 12 [DOI] 10.3389/fonc.2022.1022716. [PMID] 36338705.
2022
Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells.
Cancers. 14(5) [DOI] 10.3390/cancers14051126. [PMID] 35267434.
2022
Tumor Treating Fields Suppression of Ciliogenesis Enhances Temozolomide Toxicity.
Frontiers in oncology. 12 [DOI] 10.3389/fonc.2022.837589. [PMID] 35359402.
2021
HDAC6 Signaling at Primary Cilia Promotes Proliferation and Restricts Differentiation of Glioma Cells.
Cancers. 13(7) [DOI] 10.3390/cancers13071644. [PMID] 33915983.
2020
CCR2 inhibition reduces tumor myeloid cells and unmasks a checkpoint inhibitor effect to slow progression of resistant murine gliomas.
Proceedings of the National Academy of Sciences of the United States of America. 117(2):1129-1138 [DOI] 10.1073/pnas.1910856117. [PMID] 31879345.
2019
Emerging Roles of Primary Cilia in Glioma.
Frontiers in cellular neuroscience. 13 [DOI] 10.3389/fncel.2019.00055. [PMID] 30842728.
2019
In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons.
Neuroscience research. 143:44-52 [DOI] 10.1016/j.neures.2018.05.006. [PMID] 29857015.
2018
Glioma cell proliferation is enhanced in the presence of tumor-derived cilia vesicles
Cilia. 7(1) [DOI] 10.1186/s13630-018-0060-5. [PMID] 30410731.
2018
Infiltrative and drug-resistant slow-cycling cells support metabolic heterogeneity in glioblastoma.
The EMBO journal. 37(23) [DOI] 10.15252/embj.201798772. [PMID] 30322894.
2018
Personalized Tumor RNA Loaded Lipid-Nanoparticles Prime the Systemic and Intratumoral Milieu for Response to Cancer Immunotherapy.
Nano letters. 18(10):6195-6206 [DOI] 10.1021/acs.nanolett.8b02179. [PMID] 30259750.
2016
Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis.
Oncotarget. 7(6):7029-43 [DOI] 10.18632/oncotarget.6854. [PMID] 26760767.
2016
Neonatal seizures induced by pentylenetetrazol or kainic acid disrupt primary cilia growth on developing mouse cortical neurons.
Experimental neurology. 282:119-27 [DOI] 10.1016/j.expneurol.2016.05.015. [PMID] 27181411.
2016
Neuroanatomical characterization of the cellular and axonal architecture of subcortical band heterotopia in the BXD29-Tlr4lps-2J/J mouse cortex.
Neuroscience. 337:48-65 [DOI] 10.1016/j.neuroscience.2016.08.049. [PMID] 27595889.
2016
PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide.
Translational oncology. 9(5):392-402 [DOI] 10.1016/j.tranon.2016.08.006. [PMID] 27661404.
2016
Type 3 Adenylyl Cyclase and Somatostatin Receptor 3 Expression Persists in Aged Rat Neocortical and Hippocampal Neuronal Cilia.
Frontiers in aging neuroscience. 8 [DOI] 10.3389/fnagi.2016.00127. [PMID] 27303293.
2015
Influences of primary cilia on cortical morphogenesis and neuronal subtype maturation.
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry. 21(2):136-51 [DOI] 10.1177/1073858414531074. [PMID] 24740576.
2014
Detection of primary cilia in human glioblastoma.
Journal of neuro-oncology. 117(1):15-24 [DOI] 10.1007/s11060-013-1340-y. [PMID] 24510433.
2013
Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(6):2626-38 [DOI] 10.1523/JNEUROSCI.2906-12.2013. [PMID] 23392690.
2013
The ZEB1 pathway links glioblastoma initiation, invasion and chemoresistance.
EMBO molecular medicine. 5(8):1196-212 [DOI] 10.1002/emmm.201302827. [PMID] 23818228.
2012
Abnormal levels of Gadd45alpha in developing neocortex impair neurite outgrowth.
PloS one. 7(9) [DOI] 10.1371/journal.pone.0044207. [PMID] 22970179.
2012
Development and distribution of neuronal cilia in mouse neocortex.
The Journal of comparative neurology. 520(4):848-73 [DOI] 10.1002/cne.22793. [PMID] 22020803.
2012
Neuronal sensitivity to TDP-43 overexpression is dependent on timing of induction.
Acta neuropathologica. 123(6):807-23 [DOI] 10.1007/s00401-012-0979-3. [PMID] 22539017.
2011
Dcdc2 knockout mice display exacerbated developmental disruptions following knockdown of doublecortin.
Neuroscience. 190:398-408 [DOI] 10.1016/j.neuroscience.2011.06.010. [PMID] 21689730.
2011
Failed cytokinesis of neural progenitors in citron kinase-deficient rats leads to multiciliated neurons.
Cerebral cortex (New York, N.Y. : 1991). 21(2):338-44 [DOI] 10.1093/cercor/bhq099. [PMID] 20525772.
2010
Discs large 5: a new regulator of Citron kinase localization in developing neocortex: comment on: Chang Y, et al. Cell Cycle 2010; 9:1990-7.
Cell cycle (Georgetown, Tex.). 9(10) [PMID] 20505324.
2008
Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex.
Neuron. 60(2):273-84 [DOI] 10.1016/j.neuron.2008.09.026. [PMID] 18957219.
2008
Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling.
Proceedings of the National Academy of Sciences of the United States of America. 105(35):13127-32 [DOI] 10.1073/pnas.0804558105. [PMID] 18728187.
2008
The stumpy gene is required for mammalian ciliogenesis.
Proceedings of the National Academy of Sciences of the United States of America. 105(8):2853-8 [DOI] 10.1073/pnas.0712385105. [PMID] 18287022.
2008
Trouble making the first move: interpreting arrested neuronal migration in the cerebral cortex.
Trends in neurosciences. 31(2):54-61 [DOI] 10.1016/j.tins.2007.11.009. [PMID] 18201775.
2007
Citron kinase is required for postnatal neurogenesis in the hippocampus.
Developmental neuroscience. 29(1-2):113-23 [PMID] 17148954.
2007
Genetic determinants of neuronal migration in the cerebral cortex.
Novartis Foundation symposium. 288:45-53; discussion 53 [PMID] 18494251.
2006
MEKK4 signaling regulates filamin expression and neuronal migration.
Neuron. 52(5):789-801 [PMID] 17145501.
2005
Loss of mitogen-activated protein kinase kinase kinase 4 (MEKK4) results in enhanced apoptosis and defective neural tube development.
Proceedings of the National Academy of Sciences of the United States of America. 102(10):3846-51 [PMID] 15731347.
2003
Phosphatidylserine receptor is required for clearance of apoptotic cells.
Science (New York, N.Y.). 302(5650):1560-3 [PMID] 14645847.
2002
Citron-kinase, a protein essential to cytokinesis in neuronal progenitors, is deleted in the flathead mutant rat.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 22(8) [PMID] 11932363.
2001
Altered interneuron development in the cerebral cortex of the flathead mutant.
Cerebral cortex (New York, N.Y. : 1991). 11(8):734-43 [PMID] 11459763.
2001
Animal Models for Human Seizure and Epileptic Activity. Reply.
Epilepsy & behavior : E&B. 2(5):506-507 [PMID] 12609298.
2001
Overview of the Current Animal Models for Human Seizure and Epileptic Disorders.
Epilepsy & behavior : E&B. 2(3):201-216 [PMID] 12609365.
2000
Protective effects of prenatal choline supplementation on seizure-induced memory impairment.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(22) [PMID] 11069978.
1999
Characterization of seizures in the flathead rat: a new genetic model of epilepsy in early postnatal development.
Epilepsia. 40(4):394-400 [PMID] 10219263.
1999
Consequences of cortical dysplasia during development in rats.
Epilepsia. 40(5):537-44 [PMID] 10386521.
1999
Consequences of recurrent seizures during early brain development.
Neuroscience. 92(4):1443-54 [PMID] 10426498.
1999
Mossy fiber sprouting after recurrent seizures during early development in rats.
The Journal of comparative neurology. 404(4):537-53 [PMID] 9987996.
1998
A gene essential to brain growth and development maps to the distal arm of rat chromosome 12.
Neuroscience letters. 251(1):5-8 [PMID] 9714451.
1998
Comparison of valproate and phenobarbital treatment after status epilepticus in rats.
Neurology. 51(1):41-8 [PMID] 9674776.
1998
Synaptic reorganization following kainic acid-induced seizures during development.
Brain research. Developmental brain research. 107(2):169-77 [PMID] 9593878.
1997
Multiple kainic acid seizures in the immature and adult brain: ictal manifestations and long-term effects on learning and memory.
Epilepsia. 38(11):1157-66 [PMID] 9579915.
1997
Seizure-induced glutamate release in mature and immature animals: an in vivo microdialysis study.
Neuroreport. 8(8):2019-23 [PMID] 9223095.
1996
Long-term effects of excitatory amino acid antagonists NBQX and MK-801 on the developing brain.
Brain research. Developmental brain research. 95(2):256-62 [PMID] 8874901.

Grants

Jun 2023 ACTIVE
Regulation of the Tumor Immune Cell Landscape by Ciliated Glioblastoma Cells
Role: Principal Investigator
Funding: NATL INST OF HLTH NINDS
Sep 2022 ACTIVE
UF HEALTH CANCER CENTER PILOT PROJECTS GRANTS FUNDED THROUGHTHE FLORIDA CONSORTIUM OF NATIONAL CANCER INSTITUTE CENTER
Role: Faculty
Funding: UF HEALTH SHANDS HOSPITAL
Aug 2022 ACTIVE
Metabolic interactions between tumor cells and the immunce system in GBM A potential Achilles heel of GBM for novel therapeutics
Role: Co-Investigator
Funding: NATL INST OF HLTH NINDS
Jul 2022 ACTIVE
Improving TTFields Efficacy by Altering Ciliogenesis
Role: Principal Investigator
Funding: AMER ASSO FOR CANCER RESEARCH
Jul 2019 – Dec 2021
Enhancing TTFields Therapy for Glioblastoma By Dual Inhibition of HDAC6 and SIRT2
Role: Principal Investigator
Funding: AMER ASSO FOR CANCER RESEARCH
Sep 2018 ACTIVE
Targeting CCR2-expressing myeloid cells to overcome immune checkpoint inhibitor resistance in glioma
Role: Co-Investigator
Funding: NATL INST OF HLTH NINDS
Jun 2018 – Dec 2020
Primary Cilia-Derived ARL13B As a Biomarker and Promoter of?Gliomagenesis
Role: Principal Investigator
Funding: AMER BRAIN TUMOR ASSOCIATION
Jul 2017 – Feb 2019
Effects of Early Hypo- and Hyperthyroidism on Long-term Cortical Circuit Plasticity
Role: Co-Investigator
Funding: AMERICAN THYROID ASSOCIATION
Jun 2016 – Aug 2018
Investigation of the Human Glioblastoma Ciliome
Role: Principal Investigator
Funding: AMER BRAIN TUMOR ASSOCIATION
May 2015 – May 2016
Influence of neonatal seizure on neuronal ciliogenesis
Role: Principal Investigator
Funding: EPILEPSY FOU OF AMERICA
Apr 2015 – Mar 2018
Cortical Circuit Formation and Plasticity Following Neonatal Brain Injury
Role: Project Manager
Funding: NATL INST OF HLTH NINDS
Jul 2013 – Jun 2021
Florida Center for Brain Tumor Research – Statewide Brain Tumor Registry Program at the McKnight Brain Institute
Role: Project Manager
Funding: FL DEPT OF HLTH
Jan 2013 – Dec 2017
Identifying and Targeting Therapy Resistant Cells in Glioblastoma
Role: Principal Investigator
Funding: AMERICAN CANCER SOCIETY
Apr 2008 ACTIVE
Evelyn F. McKnight Brain Research Program in Age-Related Memory Loss.
Role: Project Manager
Funding: MCKNIGHT FOU

Contact Details

Phones:
Business:
(352) 273-5534
Emails:
Business:
msarkisian@ufl.edu
Addresses:
Business Mailing:
PO Box 100244
GAINESVILLE FL 32610