Brent A Reynolds

Brent A Reynolds,

Professor

Department: MD-NEUROLOGICAL SURGERY
Business Phone: (352) 273-9000

Teaching Profile

Courses Taught
2014-2015,2017-2023
GMS6065 Fundamentals of Cancer Biology
2018
GMS7980 Research for Doctoral Dissertation
2015-2016
GMS6331 Stem Cell Biology
2011-2012
GMS6029 Brain Journal Club
2022-2023
GMS6335 Advanced Stem Cell Biology: Tissue Engineering

Publications

2022
Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture.
Methods in molecular biology (Clifton, N.J.). 2389:87-94 [DOI] 10.1007/978-1-0716-1783-0_8. [PMID] 34558004.
2022
Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells.
Cancers. 14(5) [DOI] 10.3390/cancers14051126. [PMID] 35267434.
2020
Eco-oncology: Applying ecological principles to understand and manage cancer.
Ecology and evolution. 10(16):8538-8553 [DOI] 10.1002/ece3.6590. [PMID] 32884638.
2019
Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells.
PloS one. 14(1) [DOI] 10.1371/journal.pone.0210665. [PMID] 30657775.
2018
Infiltrative and drug-resistant slow-cycling cells support metabolic heterogeneity in glioblastoma.
The EMBO journal. 37(23) [DOI] 10.15252/embj.201798772. [PMID] 30322894.
2018
Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-21927-4. [PMID] 29476107.
2018
Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.
Methods in molecular biology (Clifton, N.J.). 1686:59-67 [DOI] 10.1007/978-1-4939-7371-2_4. [PMID] 29030812.
2017
Functional Subclone Profiling for Prediction of Treatment-Induced Intratumor Population Shifts and Discovery of Rational Drug Combinations in Human Glioblastoma.
Clinical cancer research : an official journal of the American Association for Cancer Research. 23(2):562-574 [DOI] 10.1158/1078-0432.CCR-15-2089. [PMID] 27521447.
2017
Perspective: Neuroregenerative Nutrition.
Advances in nutrition (Bethesda, Md.). 8(4):546-557 [DOI] 10.3945/an.117.015388. [PMID] 28710142.
2016
A Supplemented High-Fat Low-Carbohydrate Diet for the Treatment of Glioblastoma.
Clinical cancer research : an official journal of the American Association for Cancer Research. 22(10):2482-95 [DOI] 10.1158/1078-0432.CCR-15-0916. [PMID] 26631612.
2016
Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis.
Oncotarget. 7(6):7029-43 [DOI] 10.18632/oncotarget.6854. [PMID] 26760767.
2016
In Vitro Models for Neurogenesis.
Cold Spring Harbor perspectives in biology. 8(6) [DOI] 10.1101/cshperspect.a021279. [PMID] 26438595.
2016
PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide.
Translational oncology. 9(5):392-402 [DOI] 10.1016/j.tranon.2016.08.006. [PMID] 27661404.
2016
Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels.
Scientific reports. 6 [DOI] 10.1038/srep31915. [PMID] 27549983.
2016
Tayloring cell populations for neurodegenerative diseases.
Neural regeneration research. 11(10):1582-1583 [PMID] 27904487.
2016
Tissue Response to Deep Brain Stimulation and Microlesion: A Comparative Study.
Neuromodulation : journal of the International Neuromodulation Society. 19(5):451-8 [DOI] 10.1111/ner.12406. [PMID] 27018335.
2016
Transplantation of Defined Populations of Differentiated Human Neural Stem Cell Progeny.
Scientific reports. 6 [DOI] 10.1038/srep23579. [PMID] 27030542.
2015
Constitutive activation of myosin-dependent contractility sensitizes glioma tumor-initiating cells to mechanical inputs and reduces tissue invasion.
Cancer research. 75(6):1113-22 [DOI] 10.1158/0008-5472.CAN-13-3426. [PMID] 25634210.
2015
Differential connexin function enhances self-renewal in glioblastoma.
Cell reports. 11(7):1031-42 [DOI] 10.1016/j.celrep.2015.04.021. [PMID] 25959821.
2015
Functional integration of human neural precursor cells in mouse cortex.
PloS one. 10(3) [DOI] 10.1371/journal.pone.0120281. [PMID] 25763840.
2015
Neurogenesis and Precursor Cell Differences in the Dorsal and Ventral Adult Canine Hippocampus
Neuroscience letters. 593:107-113 [DOI] 10.1016/j.neulet.2015.03.017. [PMID] 25778416.
2015
Neurosphere and adherent culture conditions are equivalent for malignant glioma stem cell lines.
Anatomy & cell biology. 48(1):25-35 [DOI] 10.5115/acb.2015.48.1.25. [PMID] 25806119.
2015
VEGFR inhibitors upregulate CXCR4 in VEGF receptor-expressing glioblastoma in a TGFβR signaling-dependent manner.
Cancer letters. 360(1):60-7 [DOI] 10.1016/j.canlet.2015.02.005. [PMID] 25676691.
2014
Ado-trastuzamab emtansine associated hyponatremia and intracranial hemorrhage.
Acta oncologica (Stockholm, Sweden). 53(10):1434-6 [DOI] 10.3109/0284186X.2014.920959. [PMID] 24834794.
2014
Detection of primary cilia in human glioblastoma.
Journal of neuro-oncology. 117(1):15-24 [DOI] 10.1007/s11060-013-1340-y. [PMID] 24510433.
2014
Glioma stem cells: turpis omen in nomen? (The evil in the name?).
Journal of internal medicine. 276(1):25-40 [DOI] 10.1111/joim.12254. [PMID] 24708237.
2014
Hyperdiploid tumor cells increase phenotypic heterogeneity within Glioblastoma tumors.
Molecular bioSystems. 10(4):741-58 [DOI] 10.1039/c3mb70484j. [PMID] 24448662.
2014
Increased precursor cell proliferation after deep brain stimulation for Parkinson’s disease: a human study.
PloS one. 9(3) [DOI] 10.1371/journal.pone.0088770. [PMID] 24594681.
2014
Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD271.
Stem cells translational medicine. 3(4):470-80 [DOI] 10.5966/sctm.2013-0038. [PMID] 24604282.
2014
Microglia from neurogenic and non-neurogenic regions display differential proliferative potential and neuroblast support.
Frontiers in cellular neuroscience. 8 [DOI] 10.3389/fncel.2014.00180. [PMID] 25076873.
2014
Multiplex mapping of chromatin accessibility and DNA methylation within targeted single molecules identifies epigenetic heterogeneity in neural stem cells and glioblastoma.
Genome research. 24(2):329-39 [DOI] 10.1101/gr.161737.113. [PMID] 24105770.
2013
Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(39):15603-17 [DOI] 10.1523/JNEUROSCI.3004-12.2013. [PMID] 24068827.
2013
Controlling tumor invasion: bevacizumab and BMP4 for glioblastoma.
Future oncology (London, England). 9(9):1389-96 [DOI] 10.2217/fon.13.96. [PMID] 23980685.
2013
EphA3 maintains tumorigenicity and is a therapeutic target in glioblastoma multiforme.
Cancer cell. 23(2):238-48 [DOI] 10.1016/j.ccr.2013.01.007. [PMID] 23410976.
2013
Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells.
PloS one. 8(3) [DOI] 10.1371/journal.pone.0059750. [PMID] 23555768.
2013
Gap Junction Switching Maintains the Tumor Hierarchy in Glioblastoma
Neuro-Oncology. 15(3):210-211
2013
In vitro analysis of breast cancer cell line tumourspheres and primary human breast epithelia mammospheres demonstrates inter- and intrasphere heterogeneity.
PloS one. 8(6) [DOI] 10.1371/journal.pone.0064388. [PMID] 23750209.
2013
Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system.
Methods in molecular biology (Clifton, N.J.). 946:479-506 [DOI] 10.1007/978-1-62703-128-8_30. [PMID] 23179851.
2013
The ZEB1 pathway links glioblastoma initiation, invasion and chemoresistance.
EMBO molecular medicine. 5(8):1196-212 [DOI] 10.1002/emmm.201302827. [PMID] 23818228.
2012
Circulating Tumor Cells in Patients With Glioblastoma
. 78
2012
Deep brain stimulation and the role of astrocytes.
Molecular psychiatry. 17(2):124-31, 115 [DOI] 10.1038/mp.2011.61. [PMID] 21625231.
2012
Glioblastoma Tumours Can Harbour a Subset of Polyploid Cells That Are Resistant To Conventional Therapy and May Increase the Adaptive Capacity of Patient Tumours
Asia-Pacific Journal of Clinical Oncology. 8
2012
Growth hormone responsive neural precursor cells reside within the adult mammalian brain.
Scientific reports. 2 [DOI] 10.1038/srep00250. [PMID] 22355762.
2012
High-Throughput Chemical Screens Identify Disulfiram as An Inhibitor of Human Glioblastoma Stem Cells
Oncotarget. 3:1124-1136
2012
Identification and isolation of slow-dividing cells in human glioblastoma using carboxy fluorescein succinimidyl ester (CFSE).
Journal of visualized experiments : JoVE. (62) [DOI] 10.3791/3918. [PMID] 22565048.
2012
Proteomic comparison of mcf-7 tumoursphere and monolayer cultures.
PloS one. 7(12) [DOI] 10.1371/journal.pone.0052692. [PMID] 23285151.
2012
The EphA2 receptor drives self-renewal and tumorigenicity in stem-like tumor-propagating cells from human glioblastomas.
Cancer cell. 22(6):765-80 [DOI] 10.1016/j.ccr.2012.11.005. [PMID] 23238013.
2012
The neuroblast assay: an assay for the generation and enrichment of neuronal progenitor cells from differentiating neural stem cell progeny using flow cytometry.
Journal of visualized experiments : JoVE. (62) [DOI] 10.3791/3712. [PMID] 22546813.
2011
Chemokine receptor CXCR3 promotes growth of glioma.
Carcinogenesis. 32(2):129-37 [DOI] 10.1093/carcin/bgq224. [PMID] 21051441.
2011
Determination of somatic and cancer stem cell self-renewing symmetric division rate using sphere assays.
PloS one. 6(1) [DOI] 10.1371/journal.pone.0015844. [PMID] 21246056.
2011
ELK4 neutralization sensitizes glioblastoma to apoptosis through downregulation of the anti-apoptotic protein Mcl-1.
Neuro-oncology. 13(11):1202-12 [DOI] 10.1093/neuonc/nor119. [PMID] 21846680.
2011
Establishing embryonic mouse neural stem cell culture using the neurosphere assay.
Journal of visualized experiments : JoVE. (47) [DOI] 10.3791/2457. [PMID] 21248704.
2011
Ethynyldeoxyuridine (EdU) suppresses in vitro population expansion and in vivo tumor progression of human glioblastoma cells.
Journal of neuro-oncology. 105(3):485-98 [DOI] 10.1007/s11060-011-0621-6. [PMID] 21643840.
2011
Evidence for label-retaining tumour-initiating cells in human glioblastoma.
Brain : a journal of neurology. 134(Pt 5):1331-43 [DOI] 10.1093/brain/awr081. [PMID] 21515906.
2011
Isolation and characterization of adult neural stem cells.
Methods in molecular biology (Clifton, N.J.). 750:61-77 [DOI] 10.1007/978-1-61779-145-1_4. [PMID] 21618083.
2011
Isolation and expansion of human glioblastoma multiforme tumor cells using the neurosphere assay.
Journal of visualized experiments : JoVE. (56) [DOI] 10.3791/3633. [PMID] 22064695.
2011
Neural-colony forming cell assay: an assay to discriminate bona fide neural stem cells from neural progenitor cells.
Journal of visualized experiments : JoVE. (49) [DOI] 10.3791/2639. [PMID] 21403640.
2011
Purification of immature neuronal cells from neural stem cell progeny.
PloS one. 6(6) [DOI] 10.1371/journal.pone.0020941. [PMID] 21687800.
2011
Response of estrogen receptor-positive breast cancer tumorspheres to antiestrogen treatments.
PloS one. 6(4) [DOI] 10.1371/journal.pone.0018810. [PMID] 21533195.
2011
Sonic hedgehog and notch signaling can cooperate to regulate neurogenic divisions of neocortical progenitors.
PloS one. 6(2) [DOI] 10.1371/journal.pone.0014680. [PMID] 21379383.
2011
Stem Cell Transplantation for Ischemic Stroke
Stroke. 42(3) [DOI] 10.1161/STROKEAHA.110.604058.
2011
Targeting Polo-Like Kinase for the Treatment of Brain Tumors and Its Unique Capacity To Eliminate Cancer Stem Cells Through Sox-2 Inhibition
Neuro-Oncology. 13
2011
The cancer stem cell hypothesis: failures and pitfalls.
Neurosurgery. 68(2):531-45; discussion 545 [DOI] 10.1227/NEU.0b013e3181ff9eb5. [PMID] 21135745.
2011
The national DBS brain tissue network pilot study: need for more tissue and more standardization.
Cell and tissue banking. 12(3):219-31 [DOI] 10.1007/s10561-010-9189-1. [PMID] 20589432.
2011
The origins of glioma: E Pluribus Unum?
Glia. 59(8):1135-47 [DOI] 10.1002/glia.21143. [PMID] 21351156.
2011
Y-Box Binding Protein-1 (Yb-1) Inhibition Triggers Differentiation of Normal and Cancer Stem Cells From the Brain
Neuro-Oncology. 13
2011
YB-1 bridges neural stem cells and brain tumor-initiating cells via its roles in differentiation and cell growth.
Cancer research. 71(16):5569-78 [DOI] 10.1158/0008-5472.CAN-10-2805. [PMID] 21730024.
2010
Circulating Tumor Cells (Ctcs) in Patients With Glioblastoma Multiforme (Gbm)
Neuro-Oncology. 12
2010
Isolation and expansion of the adult mouse neural stem cells using the neurosphere assay.
Journal of visualized experiments : JoVE. (45) [DOI] 10.3791/2393. [PMID] 21113123.
2009
Brain cancer stem cells: Think twice before going flat.
Cell stem cell. 5(5):466-7; author reply 468 [DOI] 10.1016/j.stem.2009.10.017. [PMID] 19896437.
2009
Epha3 Regulates Cancer Progenitor Cell Self-Renewal and Proliferation in Glioma Neurosphere Cultures
Neuro-Oncology. 11
2009
Future use of mitocans against tumour-initiating cells?
Molecular nutrition & food research. 53(1):147-53 [DOI] 10.1002/mnfr.200800254. [PMID] 19123176.
2009
Identifying and enumerating neural stem cells: application to aging and cancer.
Progress in brain research. 175:43-51 [DOI] 10.1016/S0079-6123(09)17504-0. [PMID] 19660648.
2009
Isolation, expansion, and differentiation of adult Mammalian neural stem and progenitor cells using the neurosphere assay.
Methods in molecular biology (Clifton, N.J.). 549:91-101 [DOI] 10.1007/978-1-60327-931-4_7. [PMID] 19378198.
2008
Breast cancer stem cells: implications for therapy of breast cancer.
Breast cancer research : BCR. 10(4) [DOI] 10.1186/bcr2111. [PMID] 18671830.
2008
Bromodeoxyuridine induces senescence in neural stem and progenitor cells.
Stem cells (Dayton, Ohio). 26(12):3218-27 [DOI] 10.1634/stemcells.2008-0299. [PMID] 18802036.
2008
Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo.
Neoplasia (New York, N.Y.). 10(8):804-16 [PMID] 18680882.
2008
Comparative analysis of the frequency and distribution of stem and progenitor cells in the adult mouse brain.
Stem cells (Dayton, Ohio). 26(4):979-87 [DOI] 10.1634/stemcells.2007-0919. [PMID] 18203672.
2008
Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay.
Stem cells (Dayton, Ohio). 26(4):988-96 [DOI] 10.1634/stemcells.2007-0867. [PMID] 18218818.
2008
Purification of Neurons From Neural Stem Cells
Cell Transplantation. 17
2008
The neurosphere assay, a method under scrutiny.
Acta neuropsychiatrica. 20(1):2-8 [DOI] 10.1111/j.1601-5215.2007.00251.x. [PMID] 26953088.
2007
Ground-breaking stem-cell work has been reproduced.
Nature. 447(7142) [PMID] 17507958.
2007
Using the neurosphere assay to quantify neural stem cells in vivo.
Current pharmaceutical biotechnology. 8(3):141-5 [PMID] 17584087.
2006
Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells.
Nature. 444(7120):761-5 [PMID] 17151667.
2006
Brain tumour stem cells.
Nature reviews. Cancer. 6(6):425-36 [PMID] 16723989.
2006
Genetic regulators of myelopoiesis and leukemic signaling identified by gene profiling and linear modeling.
Journal of leukocyte biology. 80(2):433-47 [PMID] 16769770.
2006
Neural stem cell isolation and characterization.
Methods in enzymology. 419:3-23 [PMID] 17141049.
2005
Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue.
Methods in molecular biology (Clifton, N.J.). 290:265-80 [PMID] 15361668.
2005
Neural stem cells and neurospheres–re-evaluating the relationship.
Nature methods. 2(5):333-6 [PMID] 15846359.
1999
Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo.
Science (New York, N.Y.). 283(5401):534-7 [PMID] 9915700.
1996
Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell.
Developmental biology. 175(1):1-13 [PMID] 8608856.
1996
In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 16(8):2649-58 [PMID] 8786441.
1996
Is there a neural stem cell in the mammalian forebrain?
Trends in neurosciences. 19(9):387-93 [PMID] 8873356.
1996
Multipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 16(23):7599-609 [PMID] 8922416.
1995
BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 15(8):5765-78 [PMID] 7643217.
1994
Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells.
Neuron. 13(5):1071-82 [PMID] 7946346.
1993
bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells.
Neuron. 11(5):951-66 [PMID] 8240816.
1992
A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 12(11):4565-74 [PMID] 1432110.
1992
Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.
Science (New York, N.Y.). 255(5052):1707-10 [PMID] 1553558.

Grants

Apr 2019 – May 2020
Prana Therapeutics
Role: Principal Investigator
Funding: PRANA THERAPEUTICS
Sep 2017 – Aug 2020
Prana Therapeutics Research
Role: Principal Investigator
Funding: PRANA THERAPEUTICS
Mar 2017 ACTIVE
Neural and mesenchymal stem cell mediated gene therapy for Sanfilippo Syndrome B
Role: Co-Investigator
Funding: THE SANFILIPPO CHILDRENS RES FOU
Mar 2017 – Mar 2019
Neural and mesenchymal stem cell mediated gene therapy for Sanfilippo Syndrome B
Role: Co-Investigator
Funding: CURE SANFILIPPO FOU
Mar 2015 – Mar 2017
Application of Human Neural Stem Cells to the Injured Dog Spinal Cord
Role: Principal Investigator
Funding: STEPAHEAD AUSTRALIA LTD
Feb 2014 – Jan 2019
Establishment of a Human Grade IV Glioma Stem Cell Line Bank
Role: Principal Investigator
Funding: NATL INST OF HLTH NINDS
Jul 2013 – Jun 2021
Florida Center for Brain Tumor Research – Statewide Brain Tumor Registry Program at the McKnight Brain Institute
Role: Project Manager
Funding: FL DEPT OF HLTH
Jan 2013 – Dec 2017
Identifying and Targeting Therapy Resistant Cells in Glioblastoma
Role: Project Manager
Funding: AMER CANCER SOC
Sep 2009 ACTIVE
Isolation and expression of stem cells from fetal and adult mouse brain
Role: Principal Investigator
Funding: UF FOUNDATION

Contact Details

Phones:
Business:
(352) 273-9000
Addresses:
Business Mailing:
PO BOX 100265
DEPARTMENT OF NEUROSURGERY
GAINESVILLE FL 326100265
Business Street:
L2-100
Department of Neurosurgery
1149 Newell Dr.
Gainesville FL 32610