Stefan Prokop, M.D.
AST PROF
About Stefan Prokop
Stefan Prokop, M.D. joined the University of Florida in July 2019 from the University of Pennsylvania. Dr. Prokop serves as the director of the UF Neuromedicine Human Brain and Tissue bank (UF HBTB) and core leader for the Neuropathology core of the 1Florida Alzheimer’s disease research center (ADRC). He is also a Fixel scholar with the newly formed Norman Fixel Institute for Neurological Diseases. Dr. Prokop interfaces between clinical neuropathology with a focus on brain autopsies and research activities in the Center for Translational Research in Neurodegenerative Diseases (CTRND), the 1Florida ADRC and the Fixel Institute for Neurological diseases.
Prior to joining UF Dr. Prokop completed Neuropathology training in Switzerland and Germany and practiced for several years at the Charite Medical University in Berlin, one of the largest academic Neuropathology centers in Europe. He subsequently completed training in Anatomic Pathology and Neuropathology at the University of Pennsylvania, where he also served as a research fellow in the Center for Neurodegenerative Disease Research (CNDR).
Related Links:
Teaching Profile
Courses Taught
2019-2024
GMS6757 Introduction to Alzheimer’s Disease and Related Dementias: Clinical and Mechanistic Principles
2019
GMS7794 Neuroscience Seminar
2022
GMS6090 Research in Medical Sciences
2022-2024
DEN6350 General Pathology
2022
GMS6905 Independent Studies in Medical Sciences
2022-2024
GMS7979 Advanced Research
2022-2024
BMS6020 Clinical Neuroscience
2023
GMS6971 Research for Master’s Thesis
2024
GMS7980 Research for Doctoral Dissertation
Board Certifications
-
Anatomic PathologyAmerican Board of Pathology
-
NeuropathologyAmerican Board of Pathology
-
NeuropathologySwiss Medical Society (FMH)
Clinical Profile
Specialties
- Pathology, Immunology and Laboratory Medicine
Subspecialties
- Anatomic Pathology
- Neuropathology
Areas of Interest
- Alzheimer disease
- Dementia
- Frontotemporal dementia
Research Profile
Open Researcher and Contributor ID (ORCID)
0000-0002-5633-2149
Areas of Interest
- Alzheimer’s disease
- Microglia
- Neuroimmune interactions in Neurodegenerative diseases
- Neuropathology of Neurodegenerative diseases
- Parkinson’s disease
Publications
2024
Bridging the Gap: Multi-Omics Profiling of Brain Tissue in Alzheimer’s Disease and Older Controls in Multi-Ethnic Populations.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2024.04.16.589592.
[PMID] 38659743.
2024
Bridging the gap: Multi-omics profiling of brain tissue in Alzheimer’s disease and older controls in multi-ethnic populations.
Alzheimer's & dementia : the journal of the Alzheimer's Association.
[DOI] 10.1002/alz.14208.
[PMID] 39215503.
2024
Deterioration of neuroimmune homeostasis in Alzheimer’s Disease patients who survive a COVID-19 infection
Journal of Neuroinflammation.
21(1)
[DOI] 10.1186/s12974-024-03196-3.
[PMID] 39154174.
2024
Disease and brain region specific immune response profiles in neurodegenerative diseases with pure and mixed protein pathologies
Acta Neuropathologica Communications.
12(1)
[DOI] 10.1186/s40478-024-01770-7.
[PMID] 38581050.
2024
Hyperacetylation mimetics within the tau filament core inhibits prion-like propagation of misfolded tau.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2024.04.12.589253.
[PMID] 38659970.
2024
Integrative proteomics identifies a conserved Aβ amyloid responsome, novel plaque proteins, and pathology modifiers in Alzheimer’s disease.
Cell reports. Medicine.
5(8)
[DOI] 10.1016/j.xcrm.2024.101669.
[PMID] 39127040.
2024
Large-scale Deep Proteomic Analysis in Alzheimer’s Disease Brain Regions Across Race and Ethnicity.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2024.04.22.590547.
[PMID] 38712030.
2024
Neuritic Plaques — Gateways to Understanding Alzheimer’s Disease
Molecular Neurobiology.
61(5):2808-2821
[DOI] 10.1007/s12035-023-03736-7.
[PMID] 37940777.
2024
Novel Monoclonal Antibody Specific toward Amyloid-β Binds to a Unique Epitope within the N-Terminal Region
Antibodies.
13(3)
[DOI] 10.3390/antib13030068.
[PMID] 39189239.
2024
Oligomeric amyloid beta prevents myelination in a clusterin-dependent manner.
Research square.
[DOI] 10.21203/rs.3.rs-4415143/v1.
[PMID] 38853911.
2024
Substantia(l) Impacts of Contact Sport Play and Parkinsonism.
JAMA neurology.
81(9):909-911
[DOI] 10.1001/jamaneurol.2024.2162.
[PMID] 39008329.
2024
Targeted brain-specific tauopathy compromises peripheral skeletal muscle integrity and function.
Aging brain.
5
[DOI] 10.1016/j.nbas.2024.100110.
[PMID] 38419621.
2023
A C1qTNF3 collagen domain fusion chaperones diverse secreted proteins and anti-Aβ scFvs: Applications for gene therapies.
Molecular therapy. Methods & clinical development.
31
[DOI] 10.1016/j.omtm.2023.101146.
[PMID] 38027063.
2023
ApoE Alzheimer’s Disease Aβ-amyloid plaque morphology varies according to APOE isotype.
Research square.
[DOI] 10.21203/rs.3.rs-2524641/v1.
[PMID] 36798327.
2023
Aβ Amyloid Scaffolds the Accumulation of Matrisome and Additional Proteins in Alzheimer’s Disease.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2023.11.29.568318.
[PMID] 38076912.
2023
Cellular processing of α-synuclein fibrils results in distinct physiological C-terminal truncations with a major cleavage site at residue Glu 114.
The Journal of biological chemistry.
299(7)
[DOI] 10.1016/j.jbc.2023.104912.
[PMID] 37307916.
2023
Choroid plexus mis-splicing and altered cerebrospinal fluid composition in myotonic dystrophy type 1.
Brain : a journal of neurology.
146(10):4217-4232
[DOI] 10.1093/brain/awad148.
[PMID] 37143315.
2023
Creating the Pick’s disease International Consortium: Association study of MAPT H2 haplotype with risk of Pick’s disease.
medRxiv : the preprint server for health sciences.
[DOI] 10.1101/2023.04.17.23288471.
[PMID] 37163045.
2023
Fixed Time-Point Analysis Reveals Repetitive Mild Traumatic Brain Injury Effects on Resting State Functional Magnetic Resonance Imaging Connectivity and Neuro-Spatial Protein Profiles.
Journal of neurotrauma.
40(19-20):2037-2049
[DOI] 10.1089/neu.2022.0464.
[PMID] 37051703.
2023
Human tauopathy strains defined by phosphorylation in R1-R2 repeat domains of tau.
Acta neuropathologica communications.
11(1)
[DOI] 10.1186/s40478-023-01664-0.
[PMID] 37891635.
2023
Humanized APOE genotypes influence lifespan independently of tau aggregation in the P301S mouse model of tauopathy.
Acta neuropathologica communications.
11(1)
[DOI] 10.1186/s40478-023-01581-2.
[PMID] 37337279.
2023
Novel Conformation-Dependent Tau Antibodies Are Modulated by Adjacent Phosphorylation Sites.
International journal of molecular sciences.
24(18)
[DOI] 10.3390/ijms241813676.
[PMID] 37761979.
2023
Targeted brain-specific tauopathy compromises peripheral skeletal muscle integrity and function.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2023.11.17.567586.
[PMID] 38014109.
2023
The status of digital pathology and associated infrastructure within Alzheimer’s Disease Centers.
Journal of neuropathology and experimental neurology.
82(3):202-211
[DOI] 10.1093/jnen/nlac127.
[PMID] 36692179.
2023
Transformation of non-neuritic into neuritic plaques during AD progression drives cortical spread of tau pathology via regenerative failure
Acta Neuropathologica Communications.
11(1)
[DOI] 10.1186/s40478-023-01688-6.
[PMID] 38037144.
2022
Combinatorial model of amyloid β and tau reveals synergy between amyloid deposits and tangle formation.
Neuropathology and applied neurobiology.
48(2)
[DOI] 10.1111/nan.12779.
[PMID] 34825397.
2022
Deletion of Abi3/Gngt2 influences age-progressive amyloid β and tau pathologies in distinctive ways.
Alzheimer's research & therapy.
14(1)
[DOI] 10.1186/s13195-022-01044-1.
[PMID] 35897046.
2022
Neuropathology of Alzheimer’s Disease
Neurotherapeutics.
19(1):173-185
[DOI] 10.1007/s13311-021-01146-y.
[PMID] 34729690.
2022
Pathogenic tau recruits wild-type tau into brain inclusions and induces gut degeneration in transgenic SPAM mice.
Communications biology.
5(1)
[DOI] 10.1038/s42003-022-03373-1.
[PMID] 35550593.
2022
Reassessment of Neuronal Tau Distribution in Adult Human Brain and Implications for Tau Pathobiology.
Acta neuropathologica communications.
10(1)
[DOI] 10.1186/s40478-022-01394-9.
[PMID] 35765058.
2022
Soluble brain homogenates from diverse human and mouse sources preferentially seed diffuse Aβ plaque pathology when injected into newborn mouse hosts.
Free neuropathology.
3(9)
[DOI] 10.17879/freeneuropathology-2022-3766.
[PMID] 35494163.
2022
TREM2 risk variants are associated with atypical Alzheimer’s disease.
Acta neuropathologica.
144(6):1085-1102
[DOI] 10.1007/s00401-022-02495-4.
[PMID] 36112222.
2022
Unique seeding profiles and prion-like propagation of synucleinopathies are highly dependent on the host in human α-synuclein transgenic mice.
Acta neuropathologica.
143(6):663-685
[DOI] 10.1007/s00401-022-02425-4.
[PMID] 35488930.
2021
“Don’t Phos Over Tau”: recent developments in clinical biomarkers and therapies targeting tau phosphorylation in Alzheimer’s disease and other tauopathies.
Molecular neurodegeneration.
16(1)
[DOI] 10.1186/s13024-021-00460-5.
[PMID] 34090488.
2021
Disease-, region- and cell type specific diversity of α-synuclein carboxy terminal truncations in synucleinopathies.
Acta neuropathologica communications.
9(1)
[DOI] 10.1186/s40478-021-01242-2.
[PMID] 34454615.
2021
Robust α-synuclein pathology in select brainstem neuronal populations is a potential instigator of multiple system atrophy.
Acta neuropathologica communications.
9(1)
[DOI] 10.1186/s40478-021-01173-y.
[PMID] 33941284.
2020
Generation and Characterization of Novel Monoclonal Antibodies Targeting p62/sequestosome-1 Across Human Neurodegenerative Diseases.
Journal of neuropathology and experimental neurology.
79(4):407-418
[DOI] 10.1093/jnen/nlaa007.
[PMID] 32106300.
2020
Interleukin‐12/23 deficiency differentially affects pathology in male and female Alzheimer’s disease‐like mice
EMBO reports.
21(3)
[DOI] 10.15252/embr.201948530.
[PMID] 32003148.
2020
Novel monoclonal antibodies targeting the RRM2 domain of human TDP-43 protein.
Neuroscience letters.
738
[DOI] 10.1016/j.neulet.2020.135353.
[PMID] 32905837.
2020
Prominent amyloid plaque pathology and cerebral amyloid angiopathy in APP V717I (London) carrier – phenotypic variability in autosomal dominant Alzheimer’s disease
Acta Neuropathologica Communications.
8(1)
[DOI] 10.1186/s40478-020-0891-3.
[PMID] 32164763.
2020
Tau Ser208 phosphorylation promotes aggregation and reveals neuropathologic diversity in Alzheimer’s disease and other tauopathies.
Acta neuropathologica communications.
8(1)
[DOI] 10.1186/s40478-020-00967-w.
[PMID] 32571418.
2020
The Amyloid-beta rich CNS environment alters myeloid cell functionality independent of their origin.
Scientific reports.
10(1)
[DOI] 10.1038/s41598-020-63989-3.
[PMID] 32346002.
2019
Impact of TREM2 risk variants on brain region-specific immune activation and plaque microenvironment in Alzheimer’s disease patient brain samples
Acta Neuropathologica.
138(4):613-630
2019
Fragile X-associated tremor ataxia syndrome with co-occurrent progressive supranuclear palsy-like neuropathology
Acta Neuropathologica Communications.
7(1)
[DOI] 10.1186/s40478-019-0818-z.
[PMID] 31665069.
2019
Impact of TREM2 risk variants on brain region-specific immune activation and plaque microenvironment in Alzheimer’s disease patient brain samples
Acta Neuropathologica.
138(4):613-630
[DOI] 10.1007/s00401-019-02048-2.
[PMID] 31350575.
2019
Neuroimmune interactions in Alzheimer’s disease-New frontier with old challenges?
Progress in molecular biology and translational science.
168:183-201
[DOI] 10.1016/bs.pmbts.2019.10.002.
[PMID] 31699314.
2018
Two-Photon Fluorescence and Magnetic Resonance Specific Imaging of Aβ Amyloid Using Hybrid Nano-GdF3 Contrast Media.
ACS applied bio materials.
1(2):462-472
[DOI] 10.1021/acsabm.8b00191.
[PMID] 35016367.
2017
Immunoproteasome deficiency alters microglial cytokine response and improves cognitive deficits in Alzheimer’s disease-like APPPS1 mice
Acta Neuropathologica Communications.
5(1)
[DOI] 10.1186/s40478-017-0453-5.
[PMID] 28646899.
2016
Conserved size and periodicity of pyramidal patches in layer 2 of medial/caudal entorhinal cortex.
The Journal of comparative neurology.
524(4):783-806
[DOI] 10.1002/cne.23865.
[PMID] 26223342.
2016
High-fat diet-induced brain region-specific phenotypic spectrum of CNS resident microglia.
Acta neuropathologica.
132(3):361-75
[DOI] 10.1007/s00401-016-1595-4.
[PMID] 27393312.
2015
An azide functionalized oligothiophene ligand–a versatile tool for multimodal detection of disease associated protein aggregates.
Biosensors & bioelectronics.
63:204-211
[DOI] 10.1016/j.bios.2014.07.042.
[PMID] 25089818.
2015
Impact of peripheral myeloid cells on amyloid-β pathology in Alzheimer’s disease-like mice.
The Journal of experimental medicine.
212(11):1811-8
[DOI] 10.1084/jem.20150479.
[PMID] 26458768.
2015
Lymphocytes modulate innate immune responses and neuronal damage in experimental meningitis.
Infection and immunity.
83(1):259-67
[DOI] 10.1128/IAI.02682-14.
[PMID] 25348636.
2015
The most fulminant course of the Marburg variant of multiple sclerosis-autopsy findings.
Multiple sclerosis (Houndmills, Basingstoke, England).
21(4):485-7
[DOI] 10.1177/1352458514537366.
[PMID] 25824955.
2014
Novel γ-sarcoglycan-mutation affects cardiac function and N-terminal dystrophin expression.
Muscle & nerve.
49(1):144-5
[DOI] 10.1002/mus.23981.
[PMID] 23929688.
2014
Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription.
The Journal of biological chemistry.
289(29):20182-91
[DOI] 10.1074/jbc.M114.564690.
[PMID] 24878959.
2013
Cellular origin and diagnostic significance of high-fluorescent cells in cerebrospinal fluid detected by the XE-5000 hematology analyzer.
International journal of laboratory hematology.
35(6):580-8
[DOI] 10.1111/ijlh.12090.
[PMID] 23551597.
2013
Enhanced fluorescent assignment of protein aggregates by an oligothiophene-porphyrin-based amyloid ligand.
Macromolecular rapid communications.
34(9):723-30
[DOI] 10.1002/marc.201200817.
[PMID] 23468206.
2013
Evidence for age-dependent in vivo conformational rearrangement within Aβ amyloid deposits.
ACS chemical biology.
8(6):1128-33
[DOI] 10.1021/cb4000376.
[PMID] 23521783.
2013
Functional impairment of microglia coincides with Beta-amyloid deposition in mice with Alzheimer-like pathology.
PloS one.
8(4)
[DOI] 10.1371/journal.pone.0060921.
[PMID] 23577177.
2013
Luminescent conjugated oligothiophenes for sensitive fluorescent assignment of protein inclusion bodies.
Chembiochem : a European journal of chemical biology.
14(5):607-16
[DOI] 10.1002/cbic.201200731.
[PMID] 23450708.
2013
Microglia actions in Alzheimer’s disease.
Acta neuropathologica.
126(4):461-77
[PMID] 24224195.
2012
Inhibition of IL-12/IL-23 signaling reduces Alzheimer’s disease-like pathology and cognitive decline.
Nature medicine.
18(12):1812-9
[DOI] 10.1038/nm.2965.
[PMID] 23178247.
2011
Atypical prion protein conformation in familial prion disease with PRNP P105T mutation.
Brain pathology (Zurich, Switzerland).
21(2):209-14
[DOI] 10.1111/j.1750-3639.2010.00439.x.
[PMID] 20875062.
2011
Comparison of immunosorbent assays for the quantification of biomarkers for Alzheimer’s disease in human cerebrospinal fluid.
Dementia and geriatric cognitive disorders.
31(2):139-45
[DOI] 10.1159/000322588.
[PMID] 21304219.
2011
Impairment of immunoproteasome function by β5i/LMP7 subunit deficiency results in severe enterovirus myocarditis.
PLoS pathogens.
7(9)
[DOI] 10.1371/journal.ppat.1002233.
[PMID] 21909276.
2011
Long-term stability of Alzheimer’s disease biomarker proteins in cerebrospinal fluid.
Journal of Alzheimer's disease : JAD.
26(2):255-62
[DOI] 10.3233/JAD-2011-110329.
[PMID] 21606567.
2011
M2 polarized macrophages and giant cells contribute to myofibrosis in neuromuscular sarcoidosis.
The American journal of pathology.
178(3):1279-86
[DOI] 10.1016/j.ajpath.2010.11.065.
[PMID] 21356378.
2010
Fetal akinesia caused by a novel actin filament aggregate myopathy skeletal muscle actin gene (ACTA1) mutation.
Neuromuscular disorders : NMD.
20(8):531-3
[DOI] 10.1016/j.nmd.2010.06.008.
[PMID] 20621480.
2009
[Amyloidoses in neuropathology].
Der Pathologe.
30(3):193-6
[DOI] 10.1007/s00292-009-1131-6.
[PMID] 19340430.
2009
Formation and maintenance of Alzheimer’s disease beta-amyloid plaques in the absence of microglia.
Nature neuroscience.
12(11):1361-3
[DOI] 10.1038/nn.2432.
[PMID] 19838177.
2009
Novel pentameric thiophene derivatives for in vitro and in vivo optical imaging of a plethora of protein aggregates in cerebral amyloidoses.
ACS chemical biology.
4(8):673-84
[DOI] 10.1021/cb900112v.
[PMID] 19624097.
2005
Gamma-secretase complex assembly within the early secretory pathway.
The Journal of biological chemistry.
280(8):6471-8
[PMID] 15591316.
2005
Length and overall sequence of the PEN-2 C-terminal domain determines its function in the stabilization of presenilin fragments.
Journal of neurochemistry.
94(1):57-62
[PMID] 15953349.
2004
Identification of distinct gamma-secretase complexes with different APH-1 variants.
The Journal of biological chemistry.
279(40):41340-5
[PMID] 15286082.
2004
Requirement of PEN-2 for stabilization of the presenilin N-/C-terminal fragment heterodimer within the gamma-secretase complex.
The Journal of biological chemistry.
279(22):23255-61
[PMID] 15039426.
2002
PEN-2 is an integral component of the gamma-secretase complex required for coordinated expression of presenilin and nicastrin.
The Journal of biological chemistry.
277(42):39062-5
[PMID] 12198112.
Grants
Sep 2024
ACTIVE
Amyloidosis associated proteins in Alzheimers disease pathogenesis
Role: Principal Investigator
Funding: NATL INST OF HLTH
Sep 2022
ACTIVE
Understanding the impact of Clusterin on the oligodendrocyte lineage in AD
Role: Principal Investigator
Funding: UNIV OF VIRGINIA
via NATL INST OF HLTH NIA
Aug 2022
ACTIVE
Sepsis and the Systemic Cytokine Storm in Aging and Alzheimer Disease Models
Role: Principal Investigator
Funding: NATL INST OF HLTH NINDS
Sep 2021
– Aug 2024
Amyloidosis associated proteins in Alzheimers disease pathogenesis
Role: Principal Investigator
Funding: NATL INST OF HLTH NIA
Jul 2021
– Jun 2022
FY22 ADRC Brain Bank State of Florida Funding from MSMC
Role: Principal Investigator
Funding: MOUNT SINAI MEDICAL CTR
via FL DEPT OF ELDER AFFAIRS
Jun 2021
– Apr 2024
Combination Immunotherapy for Pediatric Brain Tumors
Role: Co-Investigator
Funding: FL DEPT OF HLTH LIVE LIKE BELLA
Apr 2021
ACTIVE
Impact of severe systemic infections on brain pathology and local immune response
Role: Principal Investigator
Funding: FL DEPT OF HLTH ED ETHEL MOORE ALZHEIMER
Apr 2021
ACTIVE
Web-based Automated Imaging Differentiation of Parkinsonism
Role: Co-Investigator
Funding: NATL INST OF HLTH NINDS
Jan 2021
– Jun 2021
FY21 ADRC Brain Bank State of Florida Funding from MSMC
Role: Principal Investigator
Funding: MOUNT SINAI MEDICAL CTR
via FL DEPT OF ELDER AFFAIRS
Dec 2020
– Dec 2022
Neoadjuvant Combination Immunotherapy for Glioblastoma
Role: Other
Funding: B*CURED
Jun 2020
ACTIVE
1Florida Alzheimers Disease Research Center
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
May 2020
ACTIVE
Biobehavioral basis of knee osteoarthritis pain
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
Jan 2020
– Dec 2020
Innate immune profiling in Lewy body dementia
Role: Principal Investigator
Funding: UF FOUNDATION
Education
Neuropathology Fellowship
2019
·
University of Pennsylvania
Anatomic Pathology Residency
2017
·
University of Pennsylvania
Neuropathology Residency
2011
·
Charite Medical University Berlin
Neuropathology Residency
2008
·
University of Zurich
Medical Degree
2005
·
Ludwig-Maximilians-University Munich
Contact Details
Phones:
- Business:
- (352) 294-5781
Emails:
- Business:
- sprokop@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100275
GAINESVILLE FL 32610