John K Neubert

John K Neubert, D.D.S.

Professor

Department: DN-ORTHODONTICS RESEARCH
Business Phone: (352) 273-5687
Business Email: jneubert@dental.ufl.edu

About John K Neubert

Dr. Neubert graduated from the University of California Santa Barbara (1990) and received his D.D.S. (1994) from the University of California Los Angles (UCLA). He has earned specialty certificates in both Orofacial Pain Management from UCLA (1996) and Orthodontics and Dentofacial Orthopedics from the University of Florida (2013). Dr. Neubert continues to treat both patients in need of orthodontics and those with orofacial pain. Dr. Neubert has been a faculty member at the University of Florida since 2003 and currently serves as the Director of pre-doctoral orthodontic education. Dr. Neubert is also an established translational researcher and his work is recognized worldwide.

Teaching Profile

Courses Taught
2018,2020-2022
DEN6940 Supervised Teaching
2011,2016-2022,2022
DEN8290 Special Topics
2011-2018,2020-2025
DEN6251 Clin Manage Dent Pain
2013-2020
DEN7819L Clinic Orthodontics 3
2012-2025
DEN7450C Orthodont Gen Pract
2011-2020
DEN5120C Physiology
2014,2018
DEN6905 Individual Study
2011-2014,2018
DEN6971 Research for Master’s Thesis
2011-2014,2018
DEN6910 Supervised Research
2015,2018
GMS6711 Neurobiology of Pain
2011,2016-2018,2018
DEN8263 Adv Oral/Med Cl Pharm
2016
DEN5013 Found Professionalism
2014
IDH4905 Individual Work
2012,2014
DEN6678 Advanced Oral Medicine and Drug Interactions in Dentistry
2011,2013-2014
DEN7540 Special Topics
2013-2014
DEN6681 Craniofacial Pathobiology
2012-2013
DEN6680 Principles and Craniofacial Biology and Emerging Therapies
2011-2012
GMS6161 Introduction to Oral Biology II
2011
GMS6160 Introduction to Oral Biology I
2011
DEN6675 Craniofacial Pain

Clinical Profile

Specialties
  • Dentistry
Subspecialties
  • Orthodontics

Publications

Academic Articles
2024
Causal interactions in brain networks predict pain levels in trigeminal neuralgia.
Brain research bulletin. 211 [DOI] 10.1016/j.brainresbull.2024.110947. [PMID] 38614409.
2024
Expectancy of alcohol analgesia moderates perception of pain relief following acute alcohol intake.
Experimental and clinical psychopharmacology. 32(2):228-235 [DOI] 10.1037/pha0000664. [PMID] 37358545.
2023
An ethogram analysis of cutaneous thermal pain sensitivity and oxycodone reward-related behaviors in rats.
Research square. [DOI] 10.21203/rs.3.rs-2679319/v1. [PMID] 36993634.
2023
An ethogram analysis of cutaneous thermal pain sensitivity and oxycodone reward-related behaviors in rats.
Scientific reports. 13(1) [DOI] 10.1038/s41598-023-36729-6. [PMID] 37380739.
2023
Imaging the neural substrate of trigeminal neuralgia pain using deep learning.
Frontiers in human neuroscience. 17 [DOI] 10.3389/fnhum.2023.1144159. [PMID] 37275345.
2023
Influence of TRPV1 on Thermal Nociception in Rats with Temporomandibular Joint Persistent Inflammation Evaluated by the Operant Orofacial Pain Assessment Device (OPAD).
Journal of pain research. 16:2047-2062 [DOI] 10.2147/JPR.S405258. [PMID] 37342611.
2022
Analgesic effects of alcohol in adults with chronic jaw pain.
Alcoholism, clinical and experimental research. 46(8):1515-1524 [DOI] 10.1111/acer.14883. [PMID] 35989585.
2022
Assessment of Nerve Injury-induced Mechanical Hypersensitivity in Rats using an Orofacial Operant Pain Assay.
Journal of visualized experiments : JoVE. (185) [DOI] 10.3791/64221. [PMID] 35969082.
2022
Effects of Oxaliplatin on Facial Sensitivity to Cool Temperatures and TRPM8 Expressing Trigeminal Ganglion Neurons in Mice.
Frontiers in pain research (Lausanne, Switzerland). 3 [DOI] 10.3389/fpain.2022.868547. [PMID] 35634452.
2022
Preclinical comparison of antinociceptive effects between ibuprofen, diclofenac, naproxen, and acetaminophen on acid-stimulated body stretching and acid-depressed feeding behaviors in rats.
Behavioural pharmacology. 33(8):567-574 [DOI] 10.1097/FBP.0000000000000704. [PMID] 36256732.
2021
Effects of bisphosphonates on different zones of the epiphyseal growth plate of rats
Research, Society and Development. 10(14) [DOI] 10.33448/rsd-v10i14.22159.
2021
Sweet-Tasting Ionic Conjugates of Local Anesthetics and Vasoconstrictors.
Molecules (Basel, Switzerland). 26(4) [DOI] 10.3390/molecules26040983. [PMID] 33673320.
2020
Pharmacological Characterization of Orofacial Nociception in Female Rats Following Nitroglycerin Administration.
Frontiers in pharmacology. 11 [DOI] 10.3389/fphar.2020.527495. [PMID] 33343340.
2018
Behavioral characteristics of capsaicin mediated cutaneous, myogenic, and arthrogenic orofacial nociception in rats.
Archives of oral biology. 92:18-24 [DOI] 10.1016/j.archoralbio.2018.04.013. [PMID] 29738922.
2018
Enoxacin and bis-enoxacin stimulate 4T1 murine breast cancer cells to release extracellular vesicles that inhibit osteoclastogenesis.
Scientific reports. 8(1) [DOI] 10.1038/s41598-018-34698-9. [PMID] 30385810.
2018
Pain control through selective chemo-axotomy of centrally projecting TRPV1+ sensory neurons.
The Journal of clinical investigation. 128(4):1657-1670 [DOI] 10.1172/JCI94331. [PMID] 29408808.
2017
Effects of cocoa-enriched diet on orofacial pain in a murine model.
Orthodontics & craniofacial research. 20 Suppl 1(Suppl 1):157-161 [DOI] 10.1111/ocr.12149. [PMID] 28643911.
2017
Effects of High-Dose Capsaicin on TMD Subjects: A Randomized Clinical Study.
JDR clinical and translational research. 2(1):58-65 [DOI] 10.1177/2380084416675837. [PMID] 28879245.
2017
Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics
Orthodontics & Craniofacial Research. 20(S1):95-99 [DOI] 10.1111/ocr.12165. [PMID] 28643924.
2017
Sex differences in mouse Transient Receptor Potential Cation Channel, Subfamily M, Member 8 expressing trigeminal ganglion neurons.
PloS one. 12(5) [DOI] 10.1371/journal.pone.0176753. [PMID] 28472061.
2016
Analgesic Activity of Tramadol and Buprenorphine after Voluntary Ingestion by Rats (Rattus norvegicus).
Journal of the American Association for Laboratory Animal Science : JAALAS. 55(1):74-82 [PMID] 26817983.
2016
Trigeminal neuroplasticity underlies allodynia in a preclinical model of mild closed head traumatic brain injury (cTBI).
Neuropharmacology. 107:27-39 [DOI] 10.1016/j.neuropharm.2016.03.016. [PMID] 26972829.
2015
A novel operant-based behavioral assay of mechanical allodynia in the orofacial region of rats.
Journal of neuroscience methods. 248:1-6 [DOI] 10.1016/j.jneumeth.2015.03.022. [PMID] 25823368.
2015
Assessment of an Orofacial Operant Pain Assay as a Preclinical Tool for Evaluating Analgesic Efficacy in Rodents.
Journal of the American Association for Laboratory Animal Science : JAALAS. 54(4):426-32 [PMID] 26224444.
2015
Ionic conjugates of lidocaine and sweeteners as better tasting local anesthetics for dentistry.
Journal of materials chemistry. B. 3(43):8492-8498 [DOI] 10.1039/c5tb00674k. [PMID] 32262689.
2015
Phosphorylation of the N-methyl-d-aspartate receptor is increased in the nucleus accumbens during both acute and extended morphine withdrawal.
The Journal of pharmacology and experimental therapeutics. 355(3):496-505 [DOI] 10.1124/jpet.115.227629. [PMID] 26377910.
2014
Gabapentin hybrid peptides and bioconjugates.
Bioorganic & medicinal chemistry. 22(4):1479-86 [DOI] 10.1016/j.bmc.2013.12.017. [PMID] 24468631.
2014
Operant assays for assessing pain in preclinical rodent models: highlights from an orofacial assay.
Current topics in behavioral neurosciences. 20:121-45 [DOI] 10.1007/7854_2014_332. [PMID] 25103871.
2014
The effects of a co-application of menthol and capsaicin on nociceptive behaviors of the rat on the operant orofacial pain assessment device.
PloS one. 9(2) [DOI] 10.1371/journal.pone.0089137. [PMID] 24558480.
2013
Anti-nociceptive effect of a conjugate of substance P and light chain of botulinum neurotoxin type A.
Pain. 154(11):2547-2553 [DOI] 10.1016/j.pain.2013.07.041. [PMID] 23933181.
2013
Assessment of the sigma-1 receptor antagonist (SR1A): a model approach for preclinical analgesic drug discovery.
European journal of pain (London, England). 17(6):789-90 [DOI] 10.1002/j.1532-2149.2013.00292.x. [PMID] 23658072.
2013
Bis-enoxacin inhibits bone resorption and orthodontic tooth movement.
Journal of dental research. 92(10):925-31 [DOI] 10.1177/0022034513501876. [PMID] 23958763.
2013
Effects of diet-induced obesity on motivation and pain behavior in an operant assay.
Neuroscience. 235:87-95 [DOI] 10.1016/j.neuroscience.2013.01.019. [PMID] 23333672.
2013
Use of the Operant Orofacial Pain Assessment Device (OPAD) to measure changes in nociceptive behavior.
Journal of visualized experiments : JoVE. (76) [DOI] 10.3791/50336. [PMID] 23792907.
2012
Characterization of bilateral trigeminal constriction injury using an operant facial pain assay.
Neuroscience. 224:294-306 [DOI] 10.1016/j.neuroscience.2012.08.015. [PMID] 22909425.
2012
Enoxacin directly inhibits osteoclastogenesis without inducing apoptosis.
The Journal of biological chemistry. 287(21):17894-17904 [DOI] 10.1074/jbc.M111.280511. [PMID] 22474295.
2012
Evaluation of An Operant Thermal Assay for the Assessment of Analgesic Efficacy in Rats
Journal of the American Association for Laboratory Animal Science. 51:638-639
2012
Intradermal injection of Botulinum toxin type A alleviates infraorbital nerve constriction-induced thermal hyperalgesia in an operant assay.
Journal of oral rehabilitation. 39(1):63-72 [DOI] 10.1111/j.1365-2842.2011.02236.x. [PMID] 21793870.
2012
Long-term changes in reward-seeking following morphine withdrawal are associated with altered N-methyl-D-aspartate receptor 1 splice variants in the amygdala.
Neuroscience. 223:45-55 [DOI] 10.1016/j.neuroscience.2012.07.037. [PMID] 22863572.
2012
Morphine and MK-801 administration leads to alternative N-methyl-D-aspartate receptor 1 splicing and associated changes in reward seeking behavior and nociception on an operant orofacial assay.
Neuroscience. 214:14-27 [DOI] 10.1016/j.neuroscience.2012.04.032. [PMID] 22531378.
2012
Placebo-induced analgesia in an operant pain model in rats.
Pain. 153(10):2009-2016 [DOI] 10.1016/j.pain.2012.04.026. [PMID] 22871471.
2011
Adaptation of a novel operant orofacial testing system to characterize both mechanical and thermal pain.
Behavioural brain research. 217(2):477-80 [DOI] 10.1016/j.bbr.2010.10.022. [PMID] 20974188.
2011
Effect of caloric and non-caloric sweet reward solutions on thermal facial operant conditioning.
Behavioural brain research. 216(2):723-5 [DOI] 10.1016/j.bbr.2010.08.023. [PMID] 20797411.
2011
Pain sensitivity and vasopressin analgesia are mediated by a gene-sex-environment interaction.
Nature neuroscience. 14(12):1569-73 [DOI] 10.1038/nn.2941. [PMID] 22019732.
2010
Central sensitization in the trigeminal nucleus caudalis produced by a conjugate of substance P and the A subunit of cholera toxin.
The journal of pain. 11(9):838-46 [DOI] 10.1016/j.jpain.2010.05.007. [PMID] 20620120.
2009
Dose-dependent effects of icilin on thermal preference in the hindpaw and face of rats.
The journal of pain. 10(6):646-53 [DOI] 10.1016/j.jpain.2009.01.002. [PMID] 19409862.
2009
Effects of hot and cold stimulus combinations on the thermal preference of rats.
Behavioural brain research. 203(2):240-6 [DOI] 10.1016/j.bbr.2009.05.009. [PMID] 19454295.
2008
Characterization of mouse orofacial pain and the effects of lesioning TRPV1-expressing neurons on operant behavior.
Molecular pain. 4 [DOI] 10.1186/1744-8069-4-43. [PMID] 18828909.
2008
Effects of environmental enrichment on thermal sensitivity in an operant orofacial pain assay.
Behavioural brain research. 187(2):478-82 [PMID] 17976833.
2008
Perineural resiniferatoxin selectively inhibits inflammatory hyperalgesia.
Molecular pain. 4 [DOI] 10.1186/1744-8069-4-3. [PMID] 18199335.
2008
Sex differences in thermal pain sensitivity and sympathetic reactivity for two strains of rat.
The journal of pain. 9(8):739-49 [DOI] 10.1016/j.jpain.2008.03.008. [PMID] 18486556.
2008
Toothache or trigeminal neuralgia: treatment dilemmas.
The journal of pain. 9(9):767-70 [DOI] 10.1016/j.jpain.2008.07.001. [PMID] 18722958.
2007
Effects of mu- and kappa-2 opioid receptor agonists on pain and rearing behaviors.
Behavioral and brain functions : BBF. 3 [PMID] 17883847.
2007
Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin.
BMC neuroscience. 8 [PMID] 17493276.
2006
Characterization of cold sensitivity and thermal preference using an operant orofacial assay.
Molecular pain. 2 [PMID] 17166284.
2006
Differentiation between capsaicin-induced allodynia and hyperalgesia using a thermal operant assay.
Behavioural brain research. 170(2):308-15 [PMID] 16675039.
2005
Peripheral targeting of the trigeminal ganglion via the infraorbital foramen as a therapeutic strategy.
Brain research. Brain research protocols. 15(3):119-26 [PMID] 16024266.
2005
Use of a novel thermal operant behavioral assay for characterization of orofacial pain sensitivity.
Pain. 116(3):386-395 [DOI] 10.1016/j.pain.2005.05.011. [PMID] 15982812.
2004
Comparison of experimental and acute clinical pain responses in humans as pain phenotypes.
The journal of pain. 5(7):377-84 [PMID] 15501195.
2004
Deletion of vanilloid receptor 1-expressing primary afferent neurons for pain control.
The Journal of clinical investigation. 113(9):1344-52 [PMID] 15124026.
2004
Genetic influence on variability in human acute experimental pain sensitivity associated with gender, ethnicity and psychological temperament.
Pain. 109(3):488-496 [DOI] 10.1016/j.pain.2004.02.027. [PMID] 15157710.
2003
Peripherally induced resiniferatoxin analgesia.
Pain. 104(1-2):219-28 [PMID] 12855332.
2003
Relationship between capsaicin-evoked substance P release and neurokinin 1 receptor internalization in the rat spinal cord.
Neuroscience. 118(2):535-45 [PMID] 12699788.
2002
Microdialysis in trigeminal ganglia.
Brain research. Brain research protocols. 10(2):102-8 [PMID] 12431709.
2001
Concurrent release of ATP and substance P within guinea pig trigeminal ganglia in vivo.
Brain research. 915(2):248-55 [PMID] 11595216.
2000
Inflammation-induced changes in primary afferent-evoked release of substance P within trigeminal ganglia in vivo.
Brain research. 871(2):181-91 [PMID] 10899285.
1996
Histochemical investigations on the influence of long-term altered gravity on the CNS of developing cichlid fish: results from the 2nd German Spacelab Mission D-2.
Advances in space research : the official journal of the Committee on Space Research (COSPAR). 17(6-7):281-4 [PMID] 11538630.

Grants

Sep 2023 – Aug 2024
Collaborative for REsearch to Advance TMD Evidence (CREATE)
Role: Co-Investigator
Funding: UNIV OF PITTSBURGH via NATL INST OF HLTH NIDCR
Jul 2021 – Jun 2023
Seven tesla preclinical MRI/S scanner for structural, functional and molecular imaging
Role: Other
Funding: NATL INST OF HLTH OD
Sep 2020 ACTIVE
Opioid and cannabinoid interactions in pain and reward
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDA
Jun 2019 – May 2022
Opioid and cannabinoid interactions in pain and reward
Role: Principal Investigator
Funding: UF RESEARCH
May 2019 – Apr 2022
Acute Effects of Alcohol Use on Chronic Orofacial Pain
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAAA
May 2019 ACTIVE
Evaluation of a cellular therapeutic for the treatment of trigeminal pain
Role: Principal Investigator
Funding: UF FOUNDATION via FACIAL PAIN RESEARCH FOUNDATION
Jul 2016 – Jun 2017
Operant behavioral instrument for measuring trigeminal neuropathic pain in rodent
Role: Principal Investigator
Funding: AMER ASSO OF ORTHODONTISTS FOU
Sep 2015 – Mar 2017
Operant behavioral instrument for measuring trigeminal neuropathic pain in rodent
Role: Principal Investigator
Funding: UF COLLEGE OF DENTISTRY SEED PROGRAM
Jul 2015 – Jun 2023
Effects of genetics on the expectation and experience of orthodontic pain
Role: Principal Investigator
Funding: AMER ASSO OF ORTHODONTISTS FOU
Feb 2015 ACTIVE
Mapping Towards a Cure Identification of Neurophysiologic Signatures of Trigeminal Neuralgia Pain
Role: Principal Investigator
Funding: UF FOUNDATION via FACIAL PAIN RESEARCH FOUNDATION

Contact Details

Phones:
Business:
(352) 273-5687
Emails:
Addresses:
Business Mailing:
PO Box 100444
GAINESVILLE FL 32610
Business Street:
COLLEGE OF DENTISTRY
1600 SW ARCHER RD., D7-44
DEPARTMENT OF ORTHODONTICS
GAINESVILLE FL 326100444