Dr. Habibeh Khoshbouei awarded NIH Shared Instrumentation Grant

Availability of a Nikon A1RMPsi-STORM4.0 that will be located at the University of Florida McKnight Brain Institute

We would like to announce the availability of a Nikon A1RMPsi-STORM4.0 that will be located at the University of Florida’s McKnight Brain Institute (MBI). This impressive and versatile system will be installed at the Cell and Tissue Analysis Core (CTAC).

Habibeh Khoshbouei
Habibeh Khoshbouei, PharmD, PhD
Associate Professor of Neuroscience

Dr. Khoshbouei was awarded a $600,000 Shared Instrumentation Grant through NIH to purchase a core-operated multiphoton system that will be located in the UF McKnight Brain Institute. Additional funds were received from the McKnight Brain Institute, the College of Medicine, and Office of Research to purchase this equipment costing in excess of $1M. This multiphoton/super resolution imaging system is supported by the MBI, Office of Research, College of Medicine, and an NIH Shared Instrumentation Grant (1S10OD020026) to Dr. Habibeh Khoshbouei.

The Nikon A1RMPsi-STORM4.0 imaging solution is a turnkey system with a number of unique features that will enhance the end-user’s research. Such a turnkey multiphoton and super resolution imaging solution allows UF researchers to 1) image deeper than traditional confocal limitation, 2) reconstruct super resolution structure, and also 3) utilize the system for single-photon confocal imaging.

Approximate installation date: October 19-23, 2015

Training (open to UF community): November 10-13, 2015


Some of the capabilities on this impressive and versatile system listed below:

  • High Resolution single-photon excitation confocal imaging
  • Fast confocal imaging with a resonant scanners
  • Confocal hybrid (galvano/resonant scanners) mode for advanced applications such as photostimulation
  • and photoactivation
  • Multiphoton imaging with either or both scanners with a tunable multiphoton laser (700nm-1080nm)
  • TIRF imaging with a Hamamatsu Flash 4.0 sCMOS camera for high resolution and large FOV
  • The new N-STORM 4.0 for super resolution imaging, suitable for both live-cell and fixed imaging, is
  • capable to resolve 20nm in lateral resolution and 50nm in axial resolution

A1RMPsi-STORM4.0 General system description:

  • Visible wavelengths for single-photon and TIRF imaging:
    • LU-NV single laser launch with diode lasers: 405nm(20mW), 445nm(20mW), 488nm(70mW), 515nm(40mW), 561nm(70mW), and 647nm(125mW
    • 4 channel simultaneously: 405nm/488nm/561/640nm
    • All combinations of wavelengths are possible in sequential mode!
    • Transmitted light detector for a widefield overlay in addition to the fluorescence confocal
  • Multiphoton imaging with a Spectra-Physics Mai Thai DS laser
    • This mode can integrate the 405nm laser for photoconversion!
    • Spectral detector to unmix overlapping fluors
  • Widefield imaging with the all-LED Lumencor Sola Light Engine (365nm-650nm)
    • Hamamatsu Flash 4.0 sCMOS camera (up to 500 FPS)
    • Filters available:
GFP Dual GFP/TxRed


  • TIRF imaging with LU-NV laser launch
  • N-STORM 4.0 Super Resolution
    • Hamamatsu Flash 4.0 sCMOS camera (up to 500 FPS)
    • Imaging channels (488/561/647nm
    • Resolution – 20nm lateral, 50nm axial